TY - GEN
T1 - A comparative study of the cytotoxic activities of three plants
T2 - 3rd International Conference on Digital Arts, Media and Technology, ICDAMT 2018
AU - Thaichinda, Sunisa
AU - Nararatwanchai, Thamthiwat
AU - Tancharoen, Salunya
AU - Kanekura, Takuro
AU - Higashi, Yuko
N1 - Publisher Copyright:
© 2018 IEEE.
PY - 2018/6/8
Y1 - 2018/6/8
N2 - Background-Nowadays much attention has been focus on the use of natural plant-derived therapies. The three kinds of Thai plants, Curcuma zedoaria (CZ), Pinus maritima (PM) and Curcuma xanthorrrhiza (CX) have been reported to have many biological and physiological properties and potential to be used as a medicine in many clinical heaths but its anti-cancer effect is still less well understood. Objective-To evaluate and compare the signaling cytotoxicity pathways of three kinds of Thai plants-CZ, PM, and CX through intracellular reactive oxygen species (ROS) in different dose on the skin cancer cell line A375 and HSC-5. Methods-We evaluated the cytotoxic activities of three plants-CZ, PM, and CX on human melanoma A375 cell line and squamous cell carcinoma HSC-5 cell line after their supplementation by detection of intracellular ROS using CM-H2DCFDA as the fluorescent probe. Results-We found that only PM significantly induced oxidative stress responses in term of ROS generation on both A375 cells and HSC-5 cell therefore, it was the most potentially cytotoxic effect within dose dependent. Conclusion-Bark extract from Pinus maritima has potentially cytotoxic and oxidative damage against both skin cancer cells by inducing ROS-mediated autosis.
AB - Background-Nowadays much attention has been focus on the use of natural plant-derived therapies. The three kinds of Thai plants, Curcuma zedoaria (CZ), Pinus maritima (PM) and Curcuma xanthorrrhiza (CX) have been reported to have many biological and physiological properties and potential to be used as a medicine in many clinical heaths but its anti-cancer effect is still less well understood. Objective-To evaluate and compare the signaling cytotoxicity pathways of three kinds of Thai plants-CZ, PM, and CX through intracellular reactive oxygen species (ROS) in different dose on the skin cancer cell line A375 and HSC-5. Methods-We evaluated the cytotoxic activities of three plants-CZ, PM, and CX on human melanoma A375 cell line and squamous cell carcinoma HSC-5 cell line after their supplementation by detection of intracellular ROS using CM-H2DCFDA as the fluorescent probe. Results-We found that only PM significantly induced oxidative stress responses in term of ROS generation on both A375 cells and HSC-5 cell therefore, it was the most potentially cytotoxic effect within dose dependent. Conclusion-Bark extract from Pinus maritima has potentially cytotoxic and oxidative damage against both skin cancer cells by inducing ROS-mediated autosis.
KW - Curcuma xanthorrrhiza
KW - Curcuma zedoaria
KW - Human Melanoma A375 cell line
KW - Pinus maritima
KW - Reactive oxygen species
KW - Squamous cell carcinoma HSC-5 cell line
UR - http://www.scopus.com/inward/record.url?scp=85049909917&partnerID=8YFLogxK
U2 - 10.1109/ICDAMT.2018.8376544
DO - 10.1109/ICDAMT.2018.8376544
M3 - Conference contribution
AN - SCOPUS:85049909917
T3 - 3rd International Conference on Digital Arts, Media and Technology, ICDAMT 2018
SP - 301
EP - 304
BT - 3rd International Conference on Digital Arts, Media and Technology, ICDAMT 2018
PB - Institute of Electrical and Electronics Engineers Inc.
Y2 - 25 February 2018 through 28 February 2018
ER -