TY - JOUR
T1 - A powerful in vivo alternative model in scientific research
T2 - Galleria mellonella
AU - Singkum, Pantira
AU - Suwanmanee, San
AU - Pumeesat, Potjaman
AU - Luplertlop, Natthanej
N1 - Publisher Copyright:
© 2019 Akadémiai Kiadó, Budapest
PY - 2019
Y1 - 2019
N2 - Murine models are suggested as the gold standard for scientific research, but they have many limitations of ethical and logistical concern. Then, the alternative host models have been developed to use in many aspects especially in invertebrate animals. These models are selected for many areas of research including genetics, physiology, biochemistry, evolution, disease, neurobiology, and behavior. During the past decade, Galleria mellonella has been used for several medical and scientific researches focusing on human pathogens. This model commonly used their larvae stage due to their easy to use, non-essential special tools or special technique, inexpensive, short life span, and no specific ethical requirement. Moreover, their innate immune response close similarly to mammals, which correlate with murine immunity. In this review, not only the current knowledge of characteristics and immune response of G. mellonella, and the practical use of these larvae in medical mycology research have been presented, but also the better understanding of their limitations has been provided.
AB - Murine models are suggested as the gold standard for scientific research, but they have many limitations of ethical and logistical concern. Then, the alternative host models have been developed to use in many aspects especially in invertebrate animals. These models are selected for many areas of research including genetics, physiology, biochemistry, evolution, disease, neurobiology, and behavior. During the past decade, Galleria mellonella has been used for several medical and scientific researches focusing on human pathogens. This model commonly used their larvae stage due to their easy to use, non-essential special tools or special technique, inexpensive, short life span, and no specific ethical requirement. Moreover, their innate immune response close similarly to mammals, which correlate with murine immunity. In this review, not only the current knowledge of characteristics and immune response of G. mellonella, and the practical use of these larvae in medical mycology research have been presented, but also the better understanding of their limitations has been provided.
KW - Galleria mellonella
KW - Innate immune response
KW - Melanization
UR - http://www.scopus.com/inward/record.url?scp=85063260831&partnerID=8YFLogxK
U2 - 10.1556/030.66.2019.001
DO - 10.1556/030.66.2019.001
M3 - Article
C2 - 30816806
AN - SCOPUS:85063260831
SN - 1217-8950
VL - 66
SP - 31
EP - 55
JO - Acta Microbiologica et Immunologica Hungarica
JF - Acta Microbiologica et Immunologica Hungarica
IS - 1
ER -