TY - GEN
T1 - Adhesion of Candida to vanillin-coated PMMA resin
AU - Thaweboon, Sroisiri
AU - Thaweboon, Boonyanit
AU - Kaypetch, Rattiporn
N1 - Publisher Copyright:
© 2017 Trans Tech Publications, Switzerland.
PY - 2017
Y1 - 2017
N2 - Denture stomatitis is a common clinical disorder seen among denture wearers. Candida albicans is considered to be the major cause which can adhere to and colonize the surface of denture base material made of polymethyl methacrylate (PMMA) resin. This study aims to investigate the effect of vanillin-coated PMMA resin on the adhesion of C. albicans. Heat-polymerized PMMA resin (Vertex RS, Dentimex, Netherlands) samples were prepared and coated with vanillin at various concentrations of 7, 11, and 15 mM. Sterile distilled water and 0.2% chlorhexidine gluconate solution were used to coat the surfaces in negative and positive controls, respectively. The adhesion assay was performed with 3 strains of C. albicans based on the method described by Samaranayake and MacFarlane. The numbers of adherent yeast cells on PMMA resin surfaces were counted under microscope by random selection of 20 fields at 400x magnification. It was found that all vanillincoated PMMA resin samples had significant decrease of candida adhesion (50-90% reduction) compared to the negative control. The effect was observed to be in a dose dependent manner (p<0.05). Chlorhexidine exhibited strong reduction effect (96-98% reduction). In conclusion, coating PMMA resin with 7-15 mM vanillin can inhibit candida adhesion to the surface. With further testing and development, vanillin may be used as coating agent for denture to prevent candida adhesion and reduce risk of denture stomatitis.
AB - Denture stomatitis is a common clinical disorder seen among denture wearers. Candida albicans is considered to be the major cause which can adhere to and colonize the surface of denture base material made of polymethyl methacrylate (PMMA) resin. This study aims to investigate the effect of vanillin-coated PMMA resin on the adhesion of C. albicans. Heat-polymerized PMMA resin (Vertex RS, Dentimex, Netherlands) samples were prepared and coated with vanillin at various concentrations of 7, 11, and 15 mM. Sterile distilled water and 0.2% chlorhexidine gluconate solution were used to coat the surfaces in negative and positive controls, respectively. The adhesion assay was performed with 3 strains of C. albicans based on the method described by Samaranayake and MacFarlane. The numbers of adherent yeast cells on PMMA resin surfaces were counted under microscope by random selection of 20 fields at 400x magnification. It was found that all vanillincoated PMMA resin samples had significant decrease of candida adhesion (50-90% reduction) compared to the negative control. The effect was observed to be in a dose dependent manner (p<0.05). Chlorhexidine exhibited strong reduction effect (96-98% reduction). In conclusion, coating PMMA resin with 7-15 mM vanillin can inhibit candida adhesion to the surface. With further testing and development, vanillin may be used as coating agent for denture to prevent candida adhesion and reduce risk of denture stomatitis.
KW - Candida
KW - PMMA resin
KW - Vanillin
UR - http://www.scopus.com/inward/record.url?scp=85036466768&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/MSF.909.171
DO - 10.4028/www.scientific.net/MSF.909.171
M3 - Conference contribution
AN - SCOPUS:85036466768
SN - 9783035712919
T3 - Materials Science Forum
SP - 171
EP - 176
BT - 5th Asia Conference on Mechanical and Materials Engineering - 5th ACMME 2017
A2 - Bin Abd Shukor, Mohd Hamdi
A2 - Es-Said, Omar S.
A2 - Chang, J.H.
PB - Trans Tech Publications Ltd
T2 - 5th Asia Conference on Mechanical and Materials Engineering, ACMME 2017
Y2 - 9 June 2017 through 11 June 2017
ER -