Adhesion of Candida to vanillin-coated PMMA resin

Sroisiri Thaweboon, Boonyanit Thaweboon, Rattiporn Kaypetch

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Citations (Scopus)

Abstract

Denture stomatitis is a common clinical disorder seen among denture wearers. Candida albicans is considered to be the major cause which can adhere to and colonize the surface of denture base material made of polymethyl methacrylate (PMMA) resin. This study aims to investigate the effect of vanillin-coated PMMA resin on the adhesion of C. albicans. Heat-polymerized PMMA resin (Vertex RS, Dentimex, Netherlands) samples were prepared and coated with vanillin at various concentrations of 7, 11, and 15 mM. Sterile distilled water and 0.2% chlorhexidine gluconate solution were used to coat the surfaces in negative and positive controls, respectively. The adhesion assay was performed with 3 strains of C. albicans based on the method described by Samaranayake and MacFarlane. The numbers of adherent yeast cells on PMMA resin surfaces were counted under microscope by random selection of 20 fields at 400x magnification. It was found that all vanillincoated PMMA resin samples had significant decrease of candida adhesion (50-90% reduction) compared to the negative control. The effect was observed to be in a dose dependent manner (p<0.05). Chlorhexidine exhibited strong reduction effect (96-98% reduction). In conclusion, coating PMMA resin with 7-15 mM vanillin can inhibit candida adhesion to the surface. With further testing and development, vanillin may be used as coating agent for denture to prevent candida adhesion and reduce risk of denture stomatitis.

Original languageEnglish
Title of host publication5th Asia Conference on Mechanical and Materials Engineering - 5th ACMME 2017
EditorsMohd Hamdi Bin Abd Shukor, Omar S. Es-Said, J.H. Chang
PublisherTrans Tech Publications Ltd
Pages171-176
Number of pages6
ISBN (Print)9783035712919
DOIs
Publication statusPublished - 2017
Event5th Asia Conference on Mechanical and Materials Engineering, ACMME 2017 - Tokyo, Japan
Duration: 9 Jun 201711 Jun 2017

Publication series

NameMaterials Science Forum
Volume909 MSF
ISSN (Print)0255-5476
ISSN (Electronic)1662-9752

Conference

Conference5th Asia Conference on Mechanical and Materials Engineering, ACMME 2017
Country/TerritoryJapan
CityTokyo
Period9/06/1711/06/17

Keywords

  • Candida
  • PMMA resin
  • Vanillin

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