TY - GEN
T1 - Adhesion of Streptococcus mutans on different types of orthodontic brackets
AU - Thaweboon, Sroisiri
AU - Aung, Yadana
AU - Luppanapornlarp, Suwannee
AU - Boonpratham, Supatchai
AU - Thaweboon, Boonyanit
PY - 2014
Y1 - 2014
N2 - Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic brackets commonly used in Thailand. Materials and Methods: The adhesion of S. mutans on 7 types of orthodontic brackets (Clarity, Spirit MB, Inspire Ice, 3M, Tomy, Ormco and W&H) was investigated in vitro. The saliva-coated brackets were immersed in a flat-bottomed 96-well plate containing S. mutans suspension (108 CFU/ml) and incubated at 37°C in 10% CO2 and 100 rpm for 2 hours. The adhered bacterial cells were fixed by 99% methanol and stained with 0.5% crystal violet. The quantity of adhesion was determined by measuring optical density (OD) at 590 nm. The data were analyzed with Kolmogorov-Smirnov test, Levene test, one way ANOVA and Games-Howell post hoc test. Results: Significantly higher bacteria adhesion was found on Clarity, Spirit MB, Tomy, Ormco and W&H compared to Inspire Ice and 3M Gemini. However, there were no significant differences among the different types of bracket materials (ceramic, plastics and metal). Conclusion: The amount of bacteria adhesion was independent of the type of bracket materials. The size and shape of brackets also played an important role in the amount of adhesion of microorganism.
AB - Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic brackets commonly used in Thailand. Materials and Methods: The adhesion of S. mutans on 7 types of orthodontic brackets (Clarity, Spirit MB, Inspire Ice, 3M, Tomy, Ormco and W&H) was investigated in vitro. The saliva-coated brackets were immersed in a flat-bottomed 96-well plate containing S. mutans suspension (108 CFU/ml) and incubated at 37°C in 10% CO2 and 100 rpm for 2 hours. The adhered bacterial cells were fixed by 99% methanol and stained with 0.5% crystal violet. The quantity of adhesion was determined by measuring optical density (OD) at 590 nm. The data were analyzed with Kolmogorov-Smirnov test, Levene test, one way ANOVA and Games-Howell post hoc test. Results: Significantly higher bacteria adhesion was found on Clarity, Spirit MB, Tomy, Ormco and W&H compared to Inspire Ice and 3M Gemini. However, there were no significant differences among the different types of bracket materials (ceramic, plastics and metal). Conclusion: The amount of bacteria adhesion was independent of the type of bracket materials. The size and shape of brackets also played an important role in the amount of adhesion of microorganism.
KW - Adhesion
KW - Orthodontic brackets
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=84896368603&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.893.467
DO - 10.4028/www.scientific.net/AMR.893.467
M3 - Conference contribution
AN - SCOPUS:84896368603
SN - 9783038350255
T3 - Advanced Materials Research
SP - 467
EP - 471
BT - Advanced Materials and Engineering Materials III
PB - Trans Tech Publications
T2 - 2013 3rd International Conference on Advanced Materials and Engineering Materials 2013, CAMEM 2013
Y2 - 14 December 2013 through 15 December 2013
ER -