Adhesion of Streptococcus mutans on different types of orthodontic brackets

Sroisiri Thaweboon, Yadana Aung, Suwannee Luppanapornlarp, Supatchai Boonpratham, Boonyanit Thaweboon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic brackets commonly used in Thailand. Materials and Methods: The adhesion of S. mutans on 7 types of orthodontic brackets (Clarity, Spirit MB, Inspire Ice, 3M, Tomy, Ormco and W&H) was investigated in vitro. The saliva-coated brackets were immersed in a flat-bottomed 96-well plate containing S. mutans suspension (108 CFU/ml) and incubated at 37°C in 10% CO2 and 100 rpm for 2 hours. The adhered bacterial cells were fixed by 99% methanol and stained with 0.5% crystal violet. The quantity of adhesion was determined by measuring optical density (OD) at 590 nm. The data were analyzed with Kolmogorov-Smirnov test, Levene test, one way ANOVA and Games-Howell post hoc test. Results: Significantly higher bacteria adhesion was found on Clarity, Spirit MB, Tomy, Ormco and W&H compared to Inspire Ice and 3M Gemini. However, there were no significant differences among the different types of bracket materials (ceramic, plastics and metal). Conclusion: The amount of bacteria adhesion was independent of the type of bracket materials. The size and shape of brackets also played an important role in the amount of adhesion of microorganism.

Original languageEnglish
Title of host publicationAdvanced Materials and Engineering Materials III
PublisherTrans Tech Publications
Pages467-471
Number of pages5
ISBN (Print)9783038350255
DOIs
Publication statusPublished - 2014
Event2013 3rd International Conference on Advanced Materials and Engineering Materials 2013, CAMEM 2013 - , Singapore
Duration: 14 Dec 201315 Dec 2013

Publication series

NameAdvanced Materials Research
Volume893
ISSN (Print)1022-6680

Conference

Conference2013 3rd International Conference on Advanced Materials and Engineering Materials 2013, CAMEM 2013
Country/TerritorySingapore
Period14/12/1315/12/13

Keywords

  • Adhesion
  • Orthodontic brackets
  • Streptococcus mutans

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