Adhesion of Streptococcus mutans on different types of orthodontic elastic O-rings

Yadana Aung, Suwannee Luppanapornlarp, Sroisiri Thaweboon, Supatchai Boonpratham, Surachai Dechkunakorn, Boonyanit Thaweboon

Research output: Contribution to journalConference articlepeer-review

Abstract

Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic elastic O-rings. Materials and methods: In vitro experiment, the adhesion of S. mutans on 4 types of elastic Orings [3M Unitek (USA), Dynaflex (USA), Thai elastic (Thailand), W&H (China)] were investigated. The saliva-coated samples were immersed in a flat-bottomed 96-well plate containing S. mutans suspension (108 CFU/ml) and incubated at 37 ̇C with 10% CO2 for 2 hours at 100 rpm. The adhered bacterial cells were fixed by using 99% methanol, then stained with 0.5% crystal violet. The quantity of adhesion was determined by measuring optical density at 590 nm. Surface area of each sample was calculated by using the formula [surface area= π2 (R2 - r2)] and surface roughness was investigated by scanning electron microscopy. The data were analyzed with Kolmogorov- Smirnov test, Levene test, one way ANOVA and Tukey HSD post hoc test. Results: High bacteria adhesion was found on 3M Unitek, whereas moderate adhesion was noted on Thai elastic and W&H. The lowest adhesion was found on Dynaflex. Conclusion: Surface area and surface roughness were factors that contributed to the amount of S. mutans adhesion on orthodontic elastic O-rings.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalApplied Mechanics and Materials
Volume487
DOIs
Publication statusPublished - 2014
Event2013 International Conference on Mechanical Structures and Smart Materials, ICMSSM 2013 - Xiamen, China
Duration: 16 Nov 201317 Nov 2013

Keywords

  • Adhesion
  • Elastic O-rings
  • Streptococcus mutans

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