TY - JOUR
T1 - Adhesion of Streptococcus mutans on different types of orthodontic elastic O-rings
AU - Aung, Yadana
AU - Luppanapornlarp, Suwannee
AU - Thaweboon, Sroisiri
AU - Boonpratham, Supatchai
AU - Dechkunakorn, Surachai
AU - Thaweboon, Boonyanit
PY - 2014
Y1 - 2014
N2 - Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic elastic O-rings. Materials and methods: In vitro experiment, the adhesion of S. mutans on 4 types of elastic Orings [3M Unitek (USA), Dynaflex (USA), Thai elastic (Thailand), W&H (China)] were investigated. The saliva-coated samples were immersed in a flat-bottomed 96-well plate containing S. mutans suspension (108 CFU/ml) and incubated at 37 ̇C with 10% CO2 for 2 hours at 100 rpm. The adhered bacterial cells were fixed by using 99% methanol, then stained with 0.5% crystal violet. The quantity of adhesion was determined by measuring optical density at 590 nm. Surface area of each sample was calculated by using the formula [surface area= π2 (R2 - r2)] and surface roughness was investigated by scanning electron microscopy. The data were analyzed with Kolmogorov- Smirnov test, Levene test, one way ANOVA and Tukey HSD post hoc test. Results: High bacteria adhesion was found on 3M Unitek, whereas moderate adhesion was noted on Thai elastic and W&H. The lowest adhesion was found on Dynaflex. Conclusion: Surface area and surface roughness were factors that contributed to the amount of S. mutans adhesion on orthodontic elastic O-rings.
AB - Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic elastic O-rings. Materials and methods: In vitro experiment, the adhesion of S. mutans on 4 types of elastic Orings [3M Unitek (USA), Dynaflex (USA), Thai elastic (Thailand), W&H (China)] were investigated. The saliva-coated samples were immersed in a flat-bottomed 96-well plate containing S. mutans suspension (108 CFU/ml) and incubated at 37 ̇C with 10% CO2 for 2 hours at 100 rpm. The adhered bacterial cells were fixed by using 99% methanol, then stained with 0.5% crystal violet. The quantity of adhesion was determined by measuring optical density at 590 nm. Surface area of each sample was calculated by using the formula [surface area= π2 (R2 - r2)] and surface roughness was investigated by scanning electron microscopy. The data were analyzed with Kolmogorov- Smirnov test, Levene test, one way ANOVA and Tukey HSD post hoc test. Results: High bacteria adhesion was found on 3M Unitek, whereas moderate adhesion was noted on Thai elastic and W&H. The lowest adhesion was found on Dynaflex. Conclusion: Surface area and surface roughness were factors that contributed to the amount of S. mutans adhesion on orthodontic elastic O-rings.
KW - Adhesion
KW - Elastic O-rings
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=84892685123&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMM.487.75
DO - 10.4028/www.scientific.net/AMM.487.75
M3 - Conference article
AN - SCOPUS:84892685123
SN - 1660-9336
VL - 487
SP - 75
EP - 79
JO - Applied Mechanics and Materials
JF - Applied Mechanics and Materials
T2 - 2013 International Conference on Mechanical Structures and Smart Materials, ICMSSM 2013
Y2 - 16 November 2013 through 17 November 2013
ER -