TY - GEN
T1 - Antibacterial Effect of Gold Nanoparticles Coated Dental Floss Against Cariogenic Bacteria
AU - Traitanon, Nattakan
AU - Dechkunakorn, Surachai
AU - Tantivitayakul, Pornpen
AU - Punnakitikashem, Primana
AU - Wichai, Wassana
AU - Whitis, Phetladda Pannak
AU - Anuwongnukroh, Niwat
N1 - Publisher Copyright:
© 2021 Trans Tech Publications Ltd, Switzerland.
PY - 2021
Y1 - 2021
N2 - Background: Carious lesions can occur on the proximal surfaces of the posterior teeth. Streptococcus mutans and Streptococcus sobrinus, are the main acidogenic bacteria that are commonly associated to dental caries. Interproximal cleaning is an important form of oral self-care habits, considering such areas of the dentition are easily affected by caries. Accordingly, dental floss has been used as an additional tool to enhance the quality of the cleaning process. It is reasonable that dental flossing should reduce interproximal caries risk because it is capable of removing parts of the interproximal plaque. The aim of this study was to evaluate the antibacterial activity of gold nanoparticle (AuNPs), when coated on unwaxed dental floss. Methods: Streptococcus mutans and Streptococcus sobrinus were cultured in Brain Heart Infusion (BHI) agar. Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the AuNPs with subcultures so obtained. Then, the bacteria were grown and spread on blood agar on which identical lengths (20mm) of unwaxed dental floss coated with gold nanoparticles (AuNPs) at concentration of 0.05, 0.1, and 0.5 mg/mL were placed. Control included untreated unwaxed dental floss and unwaxed dental floss with 0.2% Chlorhexidine (CHX). Five randomized sites of the inhibition zones were measured in millimeters in each concentration per floss. Means ± S.D. of the inhibition zones were calculated. Results: The MIC and MBC of AuNPs against S. sobrinus and S. mutans were 0.5 μg/mL and 1.0 μg/mL, respectively. The results indicated that gold nanoparticles (AuNPs) coated unwaxed dental floss, placed on blood agar with S. mutans and S. sobrinus demonstrated significant inhibition of bacterial growth. Gold nanoparticles (AuNPs) coated unwaxed dental floss at 0.05, 0.1, 0.5 mg/mL resulted in zones of inhibition ranging from 2.93 ± 0.30 mm to 4.71 ± 0.32 mm for S. sobrinus and 2.95 ± 1.73 mm to 5.18 ± 0.61 mm for S. mutans, respectively. Conclusion: In vitro study demonstrated that the AuNPs-coated unwaxed dental floss had antibacterial activities against cariogenic bacteria.
AB - Background: Carious lesions can occur on the proximal surfaces of the posterior teeth. Streptococcus mutans and Streptococcus sobrinus, are the main acidogenic bacteria that are commonly associated to dental caries. Interproximal cleaning is an important form of oral self-care habits, considering such areas of the dentition are easily affected by caries. Accordingly, dental floss has been used as an additional tool to enhance the quality of the cleaning process. It is reasonable that dental flossing should reduce interproximal caries risk because it is capable of removing parts of the interproximal plaque. The aim of this study was to evaluate the antibacterial activity of gold nanoparticle (AuNPs), when coated on unwaxed dental floss. Methods: Streptococcus mutans and Streptococcus sobrinus were cultured in Brain Heart Infusion (BHI) agar. Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the AuNPs with subcultures so obtained. Then, the bacteria were grown and spread on blood agar on which identical lengths (20mm) of unwaxed dental floss coated with gold nanoparticles (AuNPs) at concentration of 0.05, 0.1, and 0.5 mg/mL were placed. Control included untreated unwaxed dental floss and unwaxed dental floss with 0.2% Chlorhexidine (CHX). Five randomized sites of the inhibition zones were measured in millimeters in each concentration per floss. Means ± S.D. of the inhibition zones were calculated. Results: The MIC and MBC of AuNPs against S. sobrinus and S. mutans were 0.5 μg/mL and 1.0 μg/mL, respectively. The results indicated that gold nanoparticles (AuNPs) coated unwaxed dental floss, placed on blood agar with S. mutans and S. sobrinus demonstrated significant inhibition of bacterial growth. Gold nanoparticles (AuNPs) coated unwaxed dental floss at 0.05, 0.1, 0.5 mg/mL resulted in zones of inhibition ranging from 2.93 ± 0.30 mm to 4.71 ± 0.32 mm for S. sobrinus and 2.95 ± 1.73 mm to 5.18 ± 0.61 mm for S. mutans, respectively. Conclusion: In vitro study demonstrated that the AuNPs-coated unwaxed dental floss had antibacterial activities against cariogenic bacteria.
KW - Dental floss
KW - Gold nanoparticles (AuNPs)
KW - Streptococcus mutans
KW - Streptococcus sobrinus
UR - http://www.scopus.com/inward/record.url?scp=85122147357&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/KEM.904.293
DO - 10.4028/www.scientific.net/KEM.904.293
M3 - Conference contribution
AN - SCOPUS:85122147357
SN - 9783035718775
T3 - Key Engineering Materials
SP - 293
EP - 300
BT - Advanced Materials and Engineering Materials X - Selected peer-reviewed full text papers from the 10th ICAMEM and 7th ICMSSM, 2021
A2 - Wei, Peng Sheng
A2 - Xi, Yan Zhang
PB - Trans Tech Publications Ltd
T2 - 10th International Conference on Advanced Materials and Engineering Materials, ICAMEM 2021 and 7th International Conference on Mechanical Structures and Smart Materials, ICMSSM 2021
Y2 - 14 June 2021 through 15 June 2021
ER -