TY - GEN
T1 - Anticandidal activity of cratoxylum formosum gum and its cytotoxicity
AU - Thaweboon, Sroisiri
AU - Thaweboon, Boonyanit
AU - Dechkunakorn, Surachai
AU - Nisalak, Passiri
AU - Kaypetch, Rattiporn
PY - 2014
Y1 - 2014
N2 - Cratoxylum formosum is a plant widely distributed in mountainous area of various Asian countries. The extract prepared from the burnt bark has been used among the local people as a varnish to prevent tooth decay and other oral diseases. The aim of this study was to examine antifungal activity of C. formosum gum against Candida albicans and to evaluate its cytotoxicity. The gum prepared from the extract of C. formosum was investigated for antimicrobial activity against 3 strains of C. albicans. Inhibition of microbial growth was primarily tested by agar diffusion method. A two-fold broth dilution method was then used to determine the minimum inhibitory concentration (MIC) of the gum. Based on the MIC value, cytotoxicity test was performed on mouse fibroblasts (ATCC clone 929) using agar overlay technique. Inhibitory effect of the gum was seen against C. albicans with zones of inhibition ranging from 8.0 to 9.3 mm. MIC values were between 0.50 and 1.25 mg/mL. In term of cytotoxicity, C. formosum gum at the concentration of 20 MIC (25 mg/mL) was classified as grade 3 (moderate cytotoxicity) whereas those of 10 MIC and 1 MIC were grade 1 (slight cytotoxicity). In conclusion, the gum prepared from C. formosum extract exhibited antimicrobial activities against all the test strains of C. albicans. From the present study, it can be suggested that this plant can be used as a novel antifungal agent, effective against C. albicans infections, due to its inhibitory effects on C. albicans and acceptable biocompatibility. Further in vitro/in vivo studies should be conducted to understand the mechanisms of action and to establish the safe profile of this gum for clinical usage.
AB - Cratoxylum formosum is a plant widely distributed in mountainous area of various Asian countries. The extract prepared from the burnt bark has been used among the local people as a varnish to prevent tooth decay and other oral diseases. The aim of this study was to examine antifungal activity of C. formosum gum against Candida albicans and to evaluate its cytotoxicity. The gum prepared from the extract of C. formosum was investigated for antimicrobial activity against 3 strains of C. albicans. Inhibition of microbial growth was primarily tested by agar diffusion method. A two-fold broth dilution method was then used to determine the minimum inhibitory concentration (MIC) of the gum. Based on the MIC value, cytotoxicity test was performed on mouse fibroblasts (ATCC clone 929) using agar overlay technique. Inhibitory effect of the gum was seen against C. albicans with zones of inhibition ranging from 8.0 to 9.3 mm. MIC values were between 0.50 and 1.25 mg/mL. In term of cytotoxicity, C. formosum gum at the concentration of 20 MIC (25 mg/mL) was classified as grade 3 (moderate cytotoxicity) whereas those of 10 MIC and 1 MIC were grade 1 (slight cytotoxicity). In conclusion, the gum prepared from C. formosum extract exhibited antimicrobial activities against all the test strains of C. albicans. From the present study, it can be suggested that this plant can be used as a novel antifungal agent, effective against C. albicans infections, due to its inhibitory effects on C. albicans and acceptable biocompatibility. Further in vitro/in vivo studies should be conducted to understand the mechanisms of action and to establish the safe profile of this gum for clinical usage.
KW - Anticandidal
KW - Cratoxylum formosum
KW - Cytotoxicity
KW - Gum
UR - http://www.scopus.com/inward/record.url?scp=84904094353&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.974.394
DO - 10.4028/www.scientific.net/AMR.974.394
M3 - Conference contribution
AN - SCOPUS:84904094353
SN - 9783038351498
T3 - Advanced Materials Research
SP - 394
EP - 397
BT - Material and Manufacturing Technology V
PB - Trans Tech Publications Ltd
T2 - 5th International Conference on Material and Manufacturing Technology, ICMMT 2014
Y2 - 8 May 2014 through 9 May 2014
ER -