TY - CHAP
T1 - Antimicrobial Effect of Dental Adhesive on Cariogenic Multi-Species Biofilm
AU - Thaweboon, Sroisiri
AU - Saito, Takashi
AU - Mateekusontan, Sirilak
AU - Thaweboon, Boonyanit
N1 - Publisher Copyright:
© 2024 Trans Tech Publications Ltd, All Rights Reserved.
PY - 2024
Y1 - 2024
N2 - Dental caries, or tooth decay, is one of the most widespread chronic and multifactorial diseases affecting people worldwide. It is described as the localized destruction of tooth structure by acids produced from bacteria fermentation of edible carbohydrates. The disease process begins with inthe bacterial biofilm on the surfaces of the teeth. Streptococcus sanguinis, Sreptococcus gordonii, and Streptococcus mutans are the main organisms in the biofilm associated with health or disease conditions in the oral cavity. Streptococcus mutans is believed to be the primary cause of enamel demineralization and the development of dental caries. S. gordonii and S. sanguinis belong to a group of streptococci considered health-related commensal oral bacteria, crucial for forming a biofilm on oral hard tissues. Several types of materials with multipurpose characteristics have been incorporated into dental adhesives. Recently, the calcium salt of an acidic monomer (calcium salt of4-methacryloxyethyl trimellitic acid, or CMET) has been included in dental adhesive and demonstrated to suppress cariogenic biofilm formation of S. mutans. However, using a single-species model may not mimic the intricate antagonistic and synergistic relations that occur in oral biofilms. Therefore, the effect on cariogenic multi-species biofilm was assessed in this study. Dental adhesive consisting of CMET and 10-methacryloyloxydecyl dihydrogen calcium phosphate (MDCP)(Bio-Coat CA) was spread over the flat-bottom surface of the 96-well plate and LED light-cured. Then it was coated with sterile saliva at 37 °C for 45 min to form an acquired pellicle for micro bial attachment. The multi-species bacterial suspension containing Streptococcus mutans ATCC 25715, Streptococcus sanguinis ATCC 10556, and Streptococcus gordonii ATCC 10558 was prepared andadded to the saliva-coated well. Then, the plate was incubated at 37°C in a 5% CO2 atmosphere for24 h, 48 h, and 72 h to support the biofilm formation. The number of vital bacteria in the biofilm was determined with the WST-8 Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA).All tests were done in triplicate and repeated three times. For statistical analysis, Kruskal-Wallis and Dunn’s tests were employed. The results showed that at 24 h, 48 h, and 72 h, dental adhesive with CMET could inhibit the biofilm formation of multi-species bacteria significantly compared with controls. The percentages of biofilm inhibition were 29.1%, 34.7%, and 33.2% at 24 h, 48 h, and 72 h, respectively. Dental adhesive containing CMET displayed favorable multi-species biofilm-in habiting effects up to 72 h of biofilm growth. It is a promising adhesive for use to prevent secondary caries at the sites of restorations.
AB - Dental caries, or tooth decay, is one of the most widespread chronic and multifactorial diseases affecting people worldwide. It is described as the localized destruction of tooth structure by acids produced from bacteria fermentation of edible carbohydrates. The disease process begins with inthe bacterial biofilm on the surfaces of the teeth. Streptococcus sanguinis, Sreptococcus gordonii, and Streptococcus mutans are the main organisms in the biofilm associated with health or disease conditions in the oral cavity. Streptococcus mutans is believed to be the primary cause of enamel demineralization and the development of dental caries. S. gordonii and S. sanguinis belong to a group of streptococci considered health-related commensal oral bacteria, crucial for forming a biofilm on oral hard tissues. Several types of materials with multipurpose characteristics have been incorporated into dental adhesives. Recently, the calcium salt of an acidic monomer (calcium salt of4-methacryloxyethyl trimellitic acid, or CMET) has been included in dental adhesive and demonstrated to suppress cariogenic biofilm formation of S. mutans. However, using a single-species model may not mimic the intricate antagonistic and synergistic relations that occur in oral biofilms. Therefore, the effect on cariogenic multi-species biofilm was assessed in this study. Dental adhesive consisting of CMET and 10-methacryloyloxydecyl dihydrogen calcium phosphate (MDCP)(Bio-Coat CA) was spread over the flat-bottom surface of the 96-well plate and LED light-cured. Then it was coated with sterile saliva at 37 °C for 45 min to form an acquired pellicle for micro bial attachment. The multi-species bacterial suspension containing Streptococcus mutans ATCC 25715, Streptococcus sanguinis ATCC 10556, and Streptococcus gordonii ATCC 10558 was prepared andadded to the saliva-coated well. Then, the plate was incubated at 37°C in a 5% CO2 atmosphere for24 h, 48 h, and 72 h to support the biofilm formation. The number of vital bacteria in the biofilm was determined with the WST-8 Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA).All tests were done in triplicate and repeated three times. For statistical analysis, Kruskal-Wallis and Dunn’s tests were employed. The results showed that at 24 h, 48 h, and 72 h, dental adhesive with CMET could inhibit the biofilm formation of multi-species bacteria significantly compared with controls. The percentages of biofilm inhibition were 29.1%, 34.7%, and 33.2% at 24 h, 48 h, and 72 h, respectively. Dental adhesive containing CMET displayed favorable multi-species biofilm-in habiting effects up to 72 h of biofilm growth. It is a promising adhesive for use to prevent secondary caries at the sites of restorations.
KW - Adhesive
KW - caries
KW - multi-species biofilm
UR - http://www.scopus.com/inward/record.url?scp=85205108388&partnerID=8YFLogxK
U2 - 10.4028/p-EimZh9
DO - 10.4028/p-EimZh9
M3 - Chapter
AN - SCOPUS:85205108388
T3 - Key Engineering Materials
SP - 129
EP - 134
BT - Key Engineering Materials
PB - Trans Tech Publications Ltd
ER -