Candida albicans biofilm development under increased temperature

Potjaman Pumeesat, Watcharamat Muangkaew, Sumate Ampawong, Natthanej Luplertlop

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

C. albicans is one of the most important species of fungi known to produce bioflms on installed medical devices. The environment surrounding the fungi influences the development of the biofilm. Temperature is known to affect the yeast-to-hypha transition of C. albicans, but the impact of this factor on biofilm formation is still not understood. This study aimed to investigate the effects of temperature (42°C versus 37°C) on the formation of C. albicans biofilms. Three reference C. albicans strains were used: SC 5314, ATCC 90028, and ATCC 96901. Biofilm development was monitored in a series of time intervals, 2, 4, 6, 8, 24, and 48 h, at both 37°C and 42°C. Biofilm formation under each condition was evaluated by scanning electron microscopy, crystal violet staining, and 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)-carbonyl-2H-tetrazoliumhydroxide reduction assay. Our results demonstrated that at 42°C, tested strains of C. albicans could produce a biofilm, but the mass, thickness, and metabolic activity were lower than those of the biofilm formed at 37°C.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalNew Microbiologica
Volume40
Issue number4
Publication statusPublished - Oct 2017

Keywords

  • Biofilm
  • Candida albicans
  • Crystal violet staining
  • XTT

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