TY - JOUR
T1 - Candida albicans biofilm development under increased temperature
AU - Pumeesat, Potjaman
AU - Muangkaew, Watcharamat
AU - Ampawong, Sumate
AU - Luplertlop, Natthanej
N1 - Publisher Copyright:
© 2017 by EDIMES - Edizioni Internazionali Srl. All rights reserved.
PY - 2017/10
Y1 - 2017/10
N2 - C. albicans is one of the most important species of fungi known to produce bioflms on installed medical devices. The environment surrounding the fungi influences the development of the biofilm. Temperature is known to affect the yeast-to-hypha transition of C. albicans, but the impact of this factor on biofilm formation is still not understood. This study aimed to investigate the effects of temperature (42°C versus 37°C) on the formation of C. albicans biofilms. Three reference C. albicans strains were used: SC 5314, ATCC 90028, and ATCC 96901. Biofilm development was monitored in a series of time intervals, 2, 4, 6, 8, 24, and 48 h, at both 37°C and 42°C. Biofilm formation under each condition was evaluated by scanning electron microscopy, crystal violet staining, and 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)-carbonyl-2H-tetrazoliumhydroxide reduction assay. Our results demonstrated that at 42°C, tested strains of C. albicans could produce a biofilm, but the mass, thickness, and metabolic activity were lower than those of the biofilm formed at 37°C.
AB - C. albicans is one of the most important species of fungi known to produce bioflms on installed medical devices. The environment surrounding the fungi influences the development of the biofilm. Temperature is known to affect the yeast-to-hypha transition of C. albicans, but the impact of this factor on biofilm formation is still not understood. This study aimed to investigate the effects of temperature (42°C versus 37°C) on the formation of C. albicans biofilms. Three reference C. albicans strains were used: SC 5314, ATCC 90028, and ATCC 96901. Biofilm development was monitored in a series of time intervals, 2, 4, 6, 8, 24, and 48 h, at both 37°C and 42°C. Biofilm formation under each condition was evaluated by scanning electron microscopy, crystal violet staining, and 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino)-carbonyl-2H-tetrazoliumhydroxide reduction assay. Our results demonstrated that at 42°C, tested strains of C. albicans could produce a biofilm, but the mass, thickness, and metabolic activity were lower than those of the biofilm formed at 37°C.
KW - Biofilm
KW - Candida albicans
KW - Crystal violet staining
KW - XTT
UR - http://www.scopus.com/inward/record.url?scp=85038894919&partnerID=8YFLogxK
M3 - Article
C2 - 28825445
AN - SCOPUS:85038894919
SN - 1121-7138
VL - 40
SP - 279
EP - 283
JO - New Microbiologica
JF - New Microbiologica
IS - 4
ER -