TY - JOUR
T1 - Competition between Burkholderia pseudomallei and B. thailandensis Ecological and evolutionary microbiology
AU - Ngamdee, Wikanda
AU - Tandhavanant, Sarunporn
AU - Wikraiphat, Chanthiwa
AU - Reamtong, Onrapak
AU - Wuthiekanun, Vanaporn
AU - Salje, Jeanne
AU - Low, David A.
AU - Peacock, Sharon J.
AU - Chantratita, Narisara
N1 - Publisher Copyright:
© 2015 Ngamdee et al.; licensee BioMed Central.
PY - 2015
Y1 - 2015
N2 - Background: Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, an often fatal disease in tropical countries. Burkholderia thailandensis is a non-virulent but closely related species. Both species are soil saprophytes but are almost never isolated together. Results: We identified two mechanisms by which B. pseudomallei affects the growth of B. thailandensis. First, we found that six different isolates of B. pseudomallei inhibited the growth of B. thailandensis on LB agar plates. Second, our results indicated that 55% of isolated strains of B. pseudomallei produced a secreted compound that inhibited the motility but not the viability of B. thailandensis. Analysis showed that the active compound was a pH-sensitive and heat-labile compound, likely a protein, which may affect flagella processing or facilitate their degradation. Analysis of bacterial sequence types (STs) demonstrated an association between this and motility inhibition. The active compound was produced from B. pseudomallei during the stationary growth phase. Conclusion: Taken together, our results indicate that B. pseudomallei inhibits both the growth and motility of its close relative B. thailandensis. The latter phenomenon appears to occur via a previously unreported mechanism involving flagellar processing or degradation.
AB - Background: Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, an often fatal disease in tropical countries. Burkholderia thailandensis is a non-virulent but closely related species. Both species are soil saprophytes but are almost never isolated together. Results: We identified two mechanisms by which B. pseudomallei affects the growth of B. thailandensis. First, we found that six different isolates of B. pseudomallei inhibited the growth of B. thailandensis on LB agar plates. Second, our results indicated that 55% of isolated strains of B. pseudomallei produced a secreted compound that inhibited the motility but not the viability of B. thailandensis. Analysis showed that the active compound was a pH-sensitive and heat-labile compound, likely a protein, which may affect flagella processing or facilitate their degradation. Analysis of bacterial sequence types (STs) demonstrated an association between this and motility inhibition. The active compound was produced from B. pseudomallei during the stationary growth phase. Conclusion: Taken together, our results indicate that B. pseudomallei inhibits both the growth and motility of its close relative B. thailandensis. The latter phenomenon appears to occur via a previously unreported mechanism involving flagellar processing or degradation.
KW - B. thailandensis
KW - Burkholderia pseudomallei
KW - Competitive growth inhibition
KW - Flagella
KW - Melioidosis
KW - Swarming
UR - http://www.scopus.com/inward/record.url?scp=84924958767&partnerID=8YFLogxK
U2 - 10.1186/s12866-015-0395-7
DO - 10.1186/s12866-015-0395-7
M3 - Article
C2 - 25879538
AN - SCOPUS:84924958767
SN - 1471-2180
VL - 15
JO - BMC Microbiology
JF - BMC Microbiology
IS - 1
M1 - 56
ER -