TY - JOUR
T1 - Curcuminoid supplementation in canine diabetic mellitus and its complications using proteomic analysis
AU - Suemanotham, Namphung
AU - Photcharatinnakorn, Pongsakorn
AU - Chantong, Boonrat
AU - Buranasinsup, Shutipen
AU - Phochantachinda, Sataporn
AU - Sakcamduang, Walasinee
AU - Reamtong, Onrapak
AU - Thiangtrongjit, Tipparat
AU - Chatchaisak, Duangthip
N1 - Publisher Copyright:
Copyright © 2022 Suemanotham, Photcharatinnakorn, Chantong, Buranasinsup, Phochantachinda, Sakcamduang, Reamtong, Thiangtrongjit and Chatchaisak.
PY - 2022/12/23
Y1 - 2022/12/23
N2 - Introduction: Inflammation and oxidative stress contribute to diabetes pathogenesis and consequences. Therapeutic approaches for canine diabetes remain a challenge. Curcumin has anti-inflammatory and anti-oxidative effects and is beneficial for humans with diabetes mellitus (DM); however, data on its impact on canine diabetes is limited. This study aimed to evaluate the potential for causing adverse effects, anti-inflammatory effects, anti-oxidative effects and proteomic patterns of curcuminoid supplementation on canine DM. Methods: Altogether, 18 dogs were divided into two groups: DM (n = 6) and healthy (n = 12). Curcuminoid 250 mg was given to the DM group orally daily for 180 days. Blood and urine sample collection for hematological parameters, blood biochemistry, urinalysis, oxidative stress parameters, inflammatory markers and proteomics were performed every 6 weeks. Results and discussion: Curcuminoid supplementation with standard therapy significantly decreased oxidative stress with the increased glutathione/oxidized glutathione ratio, but cytokine levels were unaffected. According to the proteomic analysis, curcuminoid altered the expression of alpha-2-HS-glycoprotein, transthyretin, apolipoprotein A-I and apolipoprotein A-IV, suggesting that curcuminoid improves insulin sensitivity and reduces cardiovascular complications. No negative impact on clinical symptoms, kidneys or liver markers was identified. This study proposed that curcuminoids might be used as a targeted antioxidant strategy as an adjunctive treatment to minimize diabetes complications in dogs.
AB - Introduction: Inflammation and oxidative stress contribute to diabetes pathogenesis and consequences. Therapeutic approaches for canine diabetes remain a challenge. Curcumin has anti-inflammatory and anti-oxidative effects and is beneficial for humans with diabetes mellitus (DM); however, data on its impact on canine diabetes is limited. This study aimed to evaluate the potential for causing adverse effects, anti-inflammatory effects, anti-oxidative effects and proteomic patterns of curcuminoid supplementation on canine DM. Methods: Altogether, 18 dogs were divided into two groups: DM (n = 6) and healthy (n = 12). Curcuminoid 250 mg was given to the DM group orally daily for 180 days. Blood and urine sample collection for hematological parameters, blood biochemistry, urinalysis, oxidative stress parameters, inflammatory markers and proteomics were performed every 6 weeks. Results and discussion: Curcuminoid supplementation with standard therapy significantly decreased oxidative stress with the increased glutathione/oxidized glutathione ratio, but cytokine levels were unaffected. According to the proteomic analysis, curcuminoid altered the expression of alpha-2-HS-glycoprotein, transthyretin, apolipoprotein A-I and apolipoprotein A-IV, suggesting that curcuminoid improves insulin sensitivity and reduces cardiovascular complications. No negative impact on clinical symptoms, kidneys or liver markers was identified. This study proposed that curcuminoids might be used as a targeted antioxidant strategy as an adjunctive treatment to minimize diabetes complications in dogs.
KW - curcuminoids
KW - diabetes mellitus
KW - inflammation
KW - oxidative stress
KW - proteomic
UR - http://www.scopus.com/inward/record.url?scp=85145689875&partnerID=8YFLogxK
U2 - 10.3389/fvets.2022.1057972
DO - 10.3389/fvets.2022.1057972
M3 - Article
AN - SCOPUS:85145689875
SN - 2297-1769
VL - 9
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
M1 - 1057972
ER -