Abstract
BACKGROUND: It is believed that plasma membrane X- and Y-chromosome bearing sperm are different; therefore the freezing and thawing process may affect X- and Y-sperm differently. OBJECTIVE: The objective of this study was to investigate the effect of cryomedia and thawing extenders on the survival of X and Y-sperm. MATERIALS AND METHODS: Three different cryomedia and thawing extenders were compared. Viable motile sperm were separated using a swim-up technique. Real-time PCR was used to identify the sperm type. RESULTS: Using CryoA for freezing and Beltsville-Thawing-Solution (BTS) as the thawing extender yielded significantly higher numbers of viable motile Y sperm (64%) than control (48%) (P<0.01). Conversely, semen freezing with CryoC and thawing with Androstar®Plus gave a significantly lower number of viable motile Y sperm (32%) than control (51%). CONCLUSION: Our results revealed that defined combinations of cryomedia and thawing extenders significantly altered the survival ratio of frozen-thawed X-Y sperm in vitro, which has potential implications for artificial insemination.
| Original language | English |
|---|---|
| Pages (from-to) | 160-165 |
| Number of pages | 6 |
| Journal | Cryo-Letters |
| Volume | 38 |
| Issue number | 3 |
| Publication status | Published - May 2017 |
Keywords
- Cryomedia
- Sexual selection
- Sperm
- Thawing extenders