TY - JOUR
T1 - Dextran-Based Nanoparticles for Encapsulation of Ciprofloxacin
AU - Watcharadulyarat, Natsorn
AU - Rattanatayarom, Monthira
AU - Ruangsawasdi, Nisarat
AU - Hongdilokkul, Narupat
AU - Patikarnmonthon, Nisa
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2022/2/4
Y1 - 2022/2/4
N2 - Key success for regenerative endodontics is an effective removal of bacteria inside the root canal during the treatment. Ciprofloxacin (CIP) is one of the triple antibiotics commonly used as the root canal medicament. However, the rapid clearance of antibiotics causes bacterial reinfection. Nanoparticles (NPs) provide an alternative approach for antibiotics delivery system to improve the drug stability and release control. In the previous study, modified dextran was successfully formed the ester linkage with vinyl decanoate via lipase-catalyzed transesterification. The amphiphilic dextran, dextran-decanoate (Dex-D), has been synthesized with the degree of substitution of 83-88% and were applied for CIP-loaded nanoparticles. The suitable technique for producing antibiotic carriers considering by size, shape, and drug encapsulation efficiency was investigated in this work. The preparation methods were compared between nanoprecipitation and solid-in-oil-in-water (s/o/w) ion pairing technique. We found that the obtained Dex-D nanoparticles had stable monodispersed with spherical shape in both techniques. However, Dex-D nanoparticles formed by s/o/w ion pairing technique provided smaller size with better encapsulation efficiency. The antimicrobial activity of CIP-loaded Dex-D nanoparticles against oral pathogens showed satisfactory outcome with the MIC equal to 0.7 µg/mL. Also, the compatibility of nanoparticles and dental stem cells was observed in this work.
AB - Key success for regenerative endodontics is an effective removal of bacteria inside the root canal during the treatment. Ciprofloxacin (CIP) is one of the triple antibiotics commonly used as the root canal medicament. However, the rapid clearance of antibiotics causes bacterial reinfection. Nanoparticles (NPs) provide an alternative approach for antibiotics delivery system to improve the drug stability and release control. In the previous study, modified dextran was successfully formed the ester linkage with vinyl decanoate via lipase-catalyzed transesterification. The amphiphilic dextran, dextran-decanoate (Dex-D), has been synthesized with the degree of substitution of 83-88% and were applied for CIP-loaded nanoparticles. The suitable technique for producing antibiotic carriers considering by size, shape, and drug encapsulation efficiency was investigated in this work. The preparation methods were compared between nanoprecipitation and solid-in-oil-in-water (s/o/w) ion pairing technique. We found that the obtained Dex-D nanoparticles had stable monodispersed with spherical shape in both techniques. However, Dex-D nanoparticles formed by s/o/w ion pairing technique provided smaller size with better encapsulation efficiency. The antimicrobial activity of CIP-loaded Dex-D nanoparticles against oral pathogens showed satisfactory outcome with the MIC equal to 0.7 µg/mL. Also, the compatibility of nanoparticles and dental stem cells was observed in this work.
UR - http://www.scopus.com/inward/record.url?scp=85124966526&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/2175/1/012006
DO - 10.1088/1742-6596/2175/1/012006
M3 - Conference article
AN - SCOPUS:85124966526
SN - 1742-6588
VL - 2175
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012006
T2 - 21st International Union of Materials Research Societies International Conference in Asia, IUMRS-ICA 2021
Y2 - 23 February 2021 through 26 February 2021
ER -