Effect of Kaempferia parviflora on streptococcus mutans biofilm formation and its cytotoxicity

Supaporn Mala, Sroisiri Thaweboon, Pipat Luksamijarulkul, Boonyanit Thaweboon, Chayaporn Saranpuetti, Rattiporn Kaypetch

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Streptococcus mutans is the most prevalent bacterial species isolated from the human oral cavity. Its ability to form biofilms is an important factor in the pathogenesis of dental caries. Thus, the search for new antimicrobial agents, especially from plants, has been intensified. Kaempferia parviflora has been the subject of research for many pharmacological and antimicrobial activities. In this study, we evaluated the effect of ethanolic extract of K. parviflora root (0.46, 0.94, 1.87, 3.75, 7.5, 15, and 30 mg/ml) on S. mutans KPSK2 biofilm formation using crystal violet assay. Cytotoxicity was determined according to 10993-5/2009 on human gingival fibroblast by MTT assay. The results showed that K. parviflora extract could inhibit biofilm formation to approximately 62-82% at the concentrations of 0.46-30 mg/ml. In the case of cytotoxicity, no cytotoxic potential was demonstrated at concentration of ≤ 7.5 mg/ml of K. parviflora. In conclusion, K. parviflora extract is a potentially useful anti-biofilm agent against caries-associated bacteria and could be used as adjunct to other caries preventive measures.

Original languageEnglish
Title of host publicationApplied Engineering, Materials and Mechanics II
EditorsJong Wan Hu
PublisherTrans Tech Publications Ltd
Pages328-332
Number of pages5
ISBN (Print)9783035713374
DOIs
Publication statusPublished - 2018
Event3rd International Conference on Applied Engineering, Materials and Mechanics, ICAEMM 2018 - Okinawa Island, Japan
Duration: 20 Apr 201822 Apr 2018

Publication series

NameKey Engineering Materials
Volume773 KEM
ISSN (Print)1013-9826
ISSN (Electronic)1662-9795

Conference

Conference3rd International Conference on Applied Engineering, Materials and Mechanics, ICAEMM 2018
Country/TerritoryJapan
CityOkinawa Island
Period20/04/1822/04/18

Keywords

  • Cytotoxicity
  • Kaempferia parviflora
  • Streptococcus mutans

Fingerprint

Dive into the research topics of 'Effect of Kaempferia parviflora on streptococcus mutans biofilm formation and its cytotoxicity'. Together they form a unique fingerprint.

Cite this