TY - GEN
T1 - Effect of Kaempferia parviflora on streptococcus mutans biofilm formation and its cytotoxicity
AU - Mala, Supaporn
AU - Thaweboon, Sroisiri
AU - Luksamijarulkul, Pipat
AU - Thaweboon, Boonyanit
AU - Saranpuetti, Chayaporn
AU - Kaypetch, Rattiporn
N1 - Publisher Copyright:
© 2018 Trans Tech Publications, Switzerland.
PY - 2018
Y1 - 2018
N2 - Streptococcus mutans is the most prevalent bacterial species isolated from the human oral cavity. Its ability to form biofilms is an important factor in the pathogenesis of dental caries. Thus, the search for new antimicrobial agents, especially from plants, has been intensified. Kaempferia parviflora has been the subject of research for many pharmacological and antimicrobial activities. In this study, we evaluated the effect of ethanolic extract of K. parviflora root (0.46, 0.94, 1.87, 3.75, 7.5, 15, and 30 mg/ml) on S. mutans KPSK2 biofilm formation using crystal violet assay. Cytotoxicity was determined according to 10993-5/2009 on human gingival fibroblast by MTT assay. The results showed that K. parviflora extract could inhibit biofilm formation to approximately 62-82% at the concentrations of 0.46-30 mg/ml. In the case of cytotoxicity, no cytotoxic potential was demonstrated at concentration of ≤ 7.5 mg/ml of K. parviflora. In conclusion, K. parviflora extract is a potentially useful anti-biofilm agent against caries-associated bacteria and could be used as adjunct to other caries preventive measures.
AB - Streptococcus mutans is the most prevalent bacterial species isolated from the human oral cavity. Its ability to form biofilms is an important factor in the pathogenesis of dental caries. Thus, the search for new antimicrobial agents, especially from plants, has been intensified. Kaempferia parviflora has been the subject of research for many pharmacological and antimicrobial activities. In this study, we evaluated the effect of ethanolic extract of K. parviflora root (0.46, 0.94, 1.87, 3.75, 7.5, 15, and 30 mg/ml) on S. mutans KPSK2 biofilm formation using crystal violet assay. Cytotoxicity was determined according to 10993-5/2009 on human gingival fibroblast by MTT assay. The results showed that K. parviflora extract could inhibit biofilm formation to approximately 62-82% at the concentrations of 0.46-30 mg/ml. In the case of cytotoxicity, no cytotoxic potential was demonstrated at concentration of ≤ 7.5 mg/ml of K. parviflora. In conclusion, K. parviflora extract is a potentially useful anti-biofilm agent against caries-associated bacteria and could be used as adjunct to other caries preventive measures.
KW - Cytotoxicity
KW - Kaempferia parviflora
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=85052748265&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/KEM.773.328
DO - 10.4028/www.scientific.net/KEM.773.328
M3 - Conference contribution
AN - SCOPUS:85052748265
SN - 9783035713374
T3 - Key Engineering Materials
SP - 328
EP - 332
BT - Applied Engineering, Materials and Mechanics II
A2 - Hu, Jong Wan
PB - Trans Tech Publications Ltd
T2 - 3rd International Conference on Applied Engineering, Materials and Mechanics, ICAEMM 2018
Y2 - 20 April 2018 through 22 April 2018
ER -