TY - JOUR
T1 - Growth-inhibitory effects of farnesol against Scedosporium boydii and Lomentospora prolificans
AU - Pumeesat, Potjaman
AU - Wongsuk, Thanwa
AU - Muangkaew, Watcharamat
AU - Luplertlop, Natthanej
N1 - Publisher Copyright:
© 2017, SEAMEO TROPMED Network. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Scedosporium boydii and Lomentospora prolificans are filamentous fungi reported to cause infection in immunocompromized individuals. We studied the effect of farnesol to inhibit growth of S. boydii and L. prolificans by measuring colony diameter and determining minimal effective concentration (MEC). S. boydii and L. prolificans were grown on Sabouraud dextrose agar (SDA) at 37oC for 5 days. Conidia were collected and adjusted to a concentration of 104 conidia/ ml. Twenty microliters of conidia suspension was placed in each well of a six-well plate containing serial dilutions of farnesol (10 µM, 100 µM, 1,000 µM, and 10,000 µM) in SDA. Colony morphology and diameter were observed on days 1, 2, 3, and 4. Farnesol at concentrations of 1,000 µM or higher caused the colony diameter of both S. boydii and L. prolificans to be smaller than untreated controls in a dose-dependent manner. The MEC of farnesol to inhibit growth of both S. boydii and L. prolificans was 3.2 mM. This study reveals the antifungal property of farnesol against S. boydii and L. prolificans, which can be used for further study as an alternative antifungal agent against these fungal infections.
AB - Scedosporium boydii and Lomentospora prolificans are filamentous fungi reported to cause infection in immunocompromized individuals. We studied the effect of farnesol to inhibit growth of S. boydii and L. prolificans by measuring colony diameter and determining minimal effective concentration (MEC). S. boydii and L. prolificans were grown on Sabouraud dextrose agar (SDA) at 37oC for 5 days. Conidia were collected and adjusted to a concentration of 104 conidia/ ml. Twenty microliters of conidia suspension was placed in each well of a six-well plate containing serial dilutions of farnesol (10 µM, 100 µM, 1,000 µM, and 10,000 µM) in SDA. Colony morphology and diameter were observed on days 1, 2, 3, and 4. Farnesol at concentrations of 1,000 µM or higher caused the colony diameter of both S. boydii and L. prolificans to be smaller than untreated controls in a dose-dependent manner. The MEC of farnesol to inhibit growth of both S. boydii and L. prolificans was 3.2 mM. This study reveals the antifungal property of farnesol against S. boydii and L. prolificans, which can be used for further study as an alternative antifungal agent against these fungal infections.
KW - Farnesol
KW - Lomentospora prolificans
KW - Minimal effective concentration
KW - Scedosporium boydii
UR - http://www.scopus.com/inward/record.url?scp=85034574663&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85034574663
SN - 0125-1562
VL - 48
SP - 170
EP - 178
JO - Southeast Asian Journal of Tropical Medicine and Public Health
JF - Southeast Asian Journal of Tropical Medicine and Public Health
IS - 1
ER -