Growth-inhibitory effects of farnesol against Scedosporium boydii and Lomentospora prolificans

Potjaman Pumeesat, Thanwa Wongsuk, Watcharamat Muangkaew, Natthanej Luplertlop

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Scedosporium boydii and Lomentospora prolificans are filamentous fungi reported to cause infection in immunocompromized individuals. We studied the effect of farnesol to inhibit growth of S. boydii and L. prolificans by measuring colony diameter and determining minimal effective concentration (MEC). S. boydii and L. prolificans were grown on Sabouraud dextrose agar (SDA) at 37oC for 5 days. Conidia were collected and adjusted to a concentration of 104 conidia/ ml. Twenty microliters of conidia suspension was placed in each well of a six-well plate containing serial dilutions of farnesol (10 µM, 100 µM, 1,000 µM, and 10,000 µM) in SDA. Colony morphology and diameter were observed on days 1, 2, 3, and 4. Farnesol at concentrations of 1,000 µM or higher caused the colony diameter of both S. boydii and L. prolificans to be smaller than untreated controls in a dose-dependent manner. The MEC of farnesol to inhibit growth of both S. boydii and L. prolificans was 3.2 mM. This study reveals the antifungal property of farnesol against S. boydii and L. prolificans, which can be used for further study as an alternative antifungal agent against these fungal infections.

Original languageEnglish
Pages (from-to)170-178
Number of pages9
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume48
Issue number1
Publication statusPublished - 2017

Keywords

  • Farnesol
  • Lomentospora prolificans
  • Minimal effective concentration
  • Scedosporium boydii

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