TY - JOUR
T1 - Inhibitory Effect of Cratoxylum formosum Gum on Candida glabrata and Its α-mangostin Content
AU - Thaweboon, Sroisiri
AU - Thaweboon, Boonyanit
AU - Nisalak, Passiri
AU - Kaypetch, Rattiporn
N1 - Publisher Copyright:
© 2016 Owned by the authors, published by EDP Sciences.
PY - 2016/7/6
Y1 - 2016/7/6
N2 - Candida glabrata is the most common fungal species isolated in patients with severe mucosal inflammation. The high resistance to traditional antifungal therapies makes this species a growing concern in clinical settings. Cratoxylum formosum is a plant widely distributed in mountainous area of Asian countries. This study aims to examine antifungal activity of C.formosum gum against C.glabrata and its α-mangostin content. Inhibition of fungal growth was primarily tested by agar diffusion. Broth dilution method was then used to determine the minimum inhibitory concentration (MIC). The α-mangostin content was determined by high performance liquid chromatography (HPLC). Inhibitory effect of the gum was seen against C.glabrata (clinical isolate and ATCC22019) with zones of inhibition ranging from 14.3 to 10.2 mm. MIC value against C. glabrata ATCC22019 and the clinical isolate was 1.25 mg/mL. By HPLC, the α-mangostin content of C.formosum gum was determined as 4.08% (w/w). In conclusion, the anticandidal activity of C.formosum gum suggests that this plant may be a useful source for the development of a novel antifungal agent against candidal infection. Further in vitro/in vivo studies should be conducted to understand the mechanisms of action and to establish the safe profile of this gum for clinical usage.
AB - Candida glabrata is the most common fungal species isolated in patients with severe mucosal inflammation. The high resistance to traditional antifungal therapies makes this species a growing concern in clinical settings. Cratoxylum formosum is a plant widely distributed in mountainous area of Asian countries. This study aims to examine antifungal activity of C.formosum gum against C.glabrata and its α-mangostin content. Inhibition of fungal growth was primarily tested by agar diffusion. Broth dilution method was then used to determine the minimum inhibitory concentration (MIC). The α-mangostin content was determined by high performance liquid chromatography (HPLC). Inhibitory effect of the gum was seen against C.glabrata (clinical isolate and ATCC22019) with zones of inhibition ranging from 14.3 to 10.2 mm. MIC value against C. glabrata ATCC22019 and the clinical isolate was 1.25 mg/mL. By HPLC, the α-mangostin content of C.formosum gum was determined as 4.08% (w/w). In conclusion, the anticandidal activity of C.formosum gum suggests that this plant may be a useful source for the development of a novel antifungal agent against candidal infection. Further in vitro/in vivo studies should be conducted to understand the mechanisms of action and to establish the safe profile of this gum for clinical usage.
UR - http://www.scopus.com/inward/record.url?scp=84978496621&partnerID=8YFLogxK
U2 - 10.1051/matecconf/20166503004
DO - 10.1051/matecconf/20166503004
M3 - Conference article
AN - SCOPUS:84978496621
SN - 2261-236X
VL - 65
JO - MATEC Web of Conferences
JF - MATEC Web of Conferences
M1 - 3004
T2 - 1st International Conference on Nanomaterial, Semiconductor and Composite Materials, ICNSCM 2016
Y2 - 18 June 2016 through 19 June 2016
ER -