TY - JOUR
T1 - Isolation of fungal communities and identification of Scedosporium species complex with pathogenic potentials from a pigsty in Phra Nakhon Si Ayutthaya, Thailand
AU - Kitisin, Thitinan
AU - Muangkaew, Watcharamat
AU - Ampawong, Sumate
AU - Chutoam, Palatip
AU - Thanomsridetchai, Natthapaninee
AU - Tangwattanachuleeporn, Marut
AU - Sukphopetch, Passanesh
N1 - Publisher Copyright:
©2021 by EDIMES - Edizioni Internazionali Srl. All rights reserved
PY - 2021/1
Y1 - 2021/1
N2 - Soil fungal communities play an important role in regulating biogeochemical transformations, yet soil-related fungal pathogens are emerging threats to humans. Our previous studies have revealed the pathogenic Scedosporium species in soils samples from public parks with high human activities in Thailand. However, measurement and survey of soil fungal communities in other areas with high human/animal activities, such as the pigsty, are poorly determined. In this study, soil fungal pathogens from a pigsty were isolated and identified. Soil samples were collected from the surrounding drainage areas. Fungal species were identified using morphological and molecular analyses. Isolation of soil samples from the pigsty revealed at least 11 species that have been identified. The most abundant fungal species belonged to genera Aspergillus and Penicillium. Moreover, Scedo-Select III culturing and phylogenetic analysis with β-tubulin gene sequencing revealed the three environmental isolates of Scedosporium species, which were consistent with the S. apiospermum. These three Scedosporium isolates were susceptible to voriconazole and caused pathological characteristics of scedosporiosis similar to S. apiospermum in vivo. In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.
AB - Soil fungal communities play an important role in regulating biogeochemical transformations, yet soil-related fungal pathogens are emerging threats to humans. Our previous studies have revealed the pathogenic Scedosporium species in soils samples from public parks with high human activities in Thailand. However, measurement and survey of soil fungal communities in other areas with high human/animal activities, such as the pigsty, are poorly determined. In this study, soil fungal pathogens from a pigsty were isolated and identified. Soil samples were collected from the surrounding drainage areas. Fungal species were identified using morphological and molecular analyses. Isolation of soil samples from the pigsty revealed at least 11 species that have been identified. The most abundant fungal species belonged to genera Aspergillus and Penicillium. Moreover, Scedo-Select III culturing and phylogenetic analysis with β-tubulin gene sequencing revealed the three environmental isolates of Scedosporium species, which were consistent with the S. apiospermum. These three Scedosporium isolates were susceptible to voriconazole and caused pathological characteristics of scedosporiosis similar to S. apiospermum in vivo. In conclusion, our findings contribute towards a better understanding of soil-borne pathogenic fungi in the pigsty. The isolation of Scedosporium species with pathogenic potentials in the present study can be beneficial for the management of public health surveillance, epidemiologists, as well as physicians to reduce the risk of soil fungal contamination among pigsty workers.
KW - Fungal communities
KW - Pigsty
KW - Scedosporiosis
KW - Scedosporium apiospermum species complex
KW - Voriconazole
UR - http://www.scopus.com/inward/record.url?scp=85103214264&partnerID=8YFLogxK
M3 - Article
C2 - 33582822
AN - SCOPUS:85103214264
SN - 1121-7138
VL - 44
SP - 33
EP - 41
JO - New Microbiologica
JF - New Microbiologica
IS - 1
ER -