TY - GEN
T1 - Metal ion release and cytotoxicity of titanium orthodontic miniscrews
AU - Kosayadiloka, Katha
AU - Tangjit, Nathaphon
AU - Luppanapornlarp, Suwannee
AU - Santiwong, Peerapong
N1 - Publisher Copyright:
© 2017 Trans Tech Publications.
PY - 2017
Y1 - 2017
N2 - The aim of this study was to investigate the metal ion release and cytotoxicity of MU orthodontic miniscrews as well as two other brands of orthodontic miniscrews over time. Twenty-four orthodontic miniscrews were tested, divided into three groups of eight. Each sample extraction was performed following the ISO 10993-12:2012 method. Solutions were collected after 1, 7, and 30 days (T1, T2, and T3). The supernatants extracted from these three groups were added and exposed to mouse L929 fibroblastic cell line using an MTT cytotoxicity test. They were also tested for ion release by inductively coupled plasma-mass spectrometry (ICP-MS). Element analysis by energy-dispersive X-ray spectroscopy (EDS) was used to analyze the surfaces of the miniscrews. The quantification of three elements, namely, titanium (Ti), aluminum (Al), and vanadium (V) were assessed. The results indicated that there were no statistical differences between the self-made orthodontic miniscrews and those from two commercial groups (p<0.05). Throughout the testing period, the quantity of ions increased from T1 to T3. After 24 h, vanadium was the first to appear on the surface in small quantities in other two commercial groups. The self-made orthodontic miniscrews exhibited no toxic effects on living cells.
AB - The aim of this study was to investigate the metal ion release and cytotoxicity of MU orthodontic miniscrews as well as two other brands of orthodontic miniscrews over time. Twenty-four orthodontic miniscrews were tested, divided into three groups of eight. Each sample extraction was performed following the ISO 10993-12:2012 method. Solutions were collected after 1, 7, and 30 days (T1, T2, and T3). The supernatants extracted from these three groups were added and exposed to mouse L929 fibroblastic cell line using an MTT cytotoxicity test. They were also tested for ion release by inductively coupled plasma-mass spectrometry (ICP-MS). Element analysis by energy-dispersive X-ray spectroscopy (EDS) was used to analyze the surfaces of the miniscrews. The quantification of three elements, namely, titanium (Ti), aluminum (Al), and vanadium (V) were assessed. The results indicated that there were no statistical differences between the self-made orthodontic miniscrews and those from two commercial groups (p<0.05). Throughout the testing period, the quantity of ions increased from T1 to T3. After 24 h, vanadium was the first to appear on the surface in small quantities in other two commercial groups. The self-made orthodontic miniscrews exhibited no toxic effects on living cells.
KW - Biocompatibility
KW - Cytotoxicity
KW - Ion release
KW - Miniscrews
UR - http://www.scopus.com/inward/record.url?scp=85015059149&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/KEM.730.141
DO - 10.4028/www.scientific.net/KEM.730.141
M3 - Conference contribution
AN - SCOPUS:85015059149
SN - 9783038357834
T3 - Key Engineering Materials
SP - 141
EP - 147
BT - Innovative Materials
A2 - Ahn, Jung Kyu
PB - Trans Tech Publications Ltd
T2 - International Conference on Material Engineering and Application, ICMEA 2016
Y2 - 19 August 2016 through 21 August 2016
ER -