TY - JOUR
T1 - New records and DNA barcoding of deer flies, Chrysops (Diptera: Tabanidae) in Thailand
AU - Changbunjong, Tanasak
AU - Weluwanarak, Thekhawet
AU - Sedwisai, Poonyapat
AU - Ruangsittichai, Jiraporn
AU - Duvallet, Gerard
AU - Chareonviriyaphap, Theeraphap
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/10
Y1 - 2020/10
N2 - Chrysops spp. or deer flies (Diptera: Tabanidae) are hematophagous flies of medical and veterinary importance and some species are important vectors of Trypanosoma evansi, the causative agent of surra in Thailand. However, data regarding deer fly species and their molecular identification are limited. Accurate species identification will indicate the appropriate control measures. In this study, an entomological survey of deer flies from different sites in Thailand between May 2018 and June 2019 were conducted. In addition, mitochondrial cytochrome oxidase subunit I (COI) barcoding region was used for species identification. A total of 82 females were collected and 6 species were identified. Of these, three species are new records for Thailand: C. designatus, C. fuscomarginalis and C. vanderwulpi bringing the species total found in Thailand to nine. The COI sequences revealed an intraspecific divergence of 0.0%–2.65% and an interspecific divergence of 7.03%–13.47%. Phylogenetic analysis showed that all deer fly species were clearly separated into distinct clusters according to morphologically identified species. These results indicated that COI barcodes were capable in discriminating between deer fly species on the basis of the barcoding gap and phylogenetic analysis. Therefore, DNA barcoding is a valuable tool for species identification of deer flies in Thailand.
AB - Chrysops spp. or deer flies (Diptera: Tabanidae) are hematophagous flies of medical and veterinary importance and some species are important vectors of Trypanosoma evansi, the causative agent of surra in Thailand. However, data regarding deer fly species and their molecular identification are limited. Accurate species identification will indicate the appropriate control measures. In this study, an entomological survey of deer flies from different sites in Thailand between May 2018 and June 2019 were conducted. In addition, mitochondrial cytochrome oxidase subunit I (COI) barcoding region was used for species identification. A total of 82 females were collected and 6 species were identified. Of these, three species are new records for Thailand: C. designatus, C. fuscomarginalis and C. vanderwulpi bringing the species total found in Thailand to nine. The COI sequences revealed an intraspecific divergence of 0.0%–2.65% and an interspecific divergence of 7.03%–13.47%. Phylogenetic analysis showed that all deer fly species were clearly separated into distinct clusters according to morphologically identified species. These results indicated that COI barcodes were capable in discriminating between deer fly species on the basis of the barcoding gap and phylogenetic analysis. Therefore, DNA barcoding is a valuable tool for species identification of deer flies in Thailand.
KW - Cytochrome oxidase I
KW - DNA barcode
KW - Morphology
KW - Tabanids
KW - Trypanosoma
KW - Vectors
UR - http://www.scopus.com/inward/record.url?scp=85086118811&partnerID=8YFLogxK
U2 - 10.1016/j.actatropica.2020.105532
DO - 10.1016/j.actatropica.2020.105532
M3 - Article
C2 - 32497542
AN - SCOPUS:85086118811
SN - 0001-706X
VL - 210
JO - Acta Tropica
JF - Acta Tropica
M1 - 105532
ER -