TY - JOUR
T1 - Opisthorchis viverrini
T2 - Identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis
AU - Ruangsittichai, Jiraporn
AU - Viyanant, Vithoon
AU - Vichasri-Grams, Suksiri
AU - Sobhon, Prasert
AU - Tesana, Smarn
AU - Upatham, Edward Suchart
AU - Hofmann, Annemarie
AU - Korge, Günter
AU - Grams, Rudi
PY - 2006/11
Y1 - 2006/11
N2 - A cDNA encoding a novel eggshell protein (OvESP) with high-glycine (49.2%) and -tyrosine (27.8%) content was cloned from the human liver fluke Opisthorchis viverrini. In the adult parasite, the RNA products of the OvESP gene are limited to the vitelline follicles. They have a size of 800 nucleotides and are already present in 2-week-old juveniles. Immune sera of hamsters, experimentally infected, and humans, naturally infected with O. viverrini, detect bacterially expressed recombinant OvESP (rOvESP). A rabbit anti-rOvESP antiserum only reacts with the shells of intrauterine eggs in tissue sections of the parasite. Comparison of rOvESP with the parasite's excretion/secretion products as diagnostic tools for human opisthorchiasis shows a higher sensitivity (0.82-0.48) and specificity (0.97-0.91) of the recombinant protein in the ELISA technique. But the observed weak cross-reactivity of immune sera from mice infected with Schistosoma mansoni, Schistosoma mekongi, and Fasciola gigantica in Western blots of rOvESP indicates that the diagnostic quality of this protein might be compromised if infections by other trematodes are present.
AB - A cDNA encoding a novel eggshell protein (OvESP) with high-glycine (49.2%) and -tyrosine (27.8%) content was cloned from the human liver fluke Opisthorchis viverrini. In the adult parasite, the RNA products of the OvESP gene are limited to the vitelline follicles. They have a size of 800 nucleotides and are already present in 2-week-old juveniles. Immune sera of hamsters, experimentally infected, and humans, naturally infected with O. viverrini, detect bacterially expressed recombinant OvESP (rOvESP). A rabbit anti-rOvESP antiserum only reacts with the shells of intrauterine eggs in tissue sections of the parasite. Comparison of rOvESP with the parasite's excretion/secretion products as diagnostic tools for human opisthorchiasis shows a higher sensitivity (0.82-0.48) and specificity (0.97-0.91) of the recombinant protein in the ELISA technique. But the observed weak cross-reactivity of immune sera from mice infected with Schistosoma mansoni, Schistosoma mekongi, and Fasciola gigantica in Western blots of rOvESP indicates that the diagnostic quality of this protein might be compromised if infections by other trematodes are present.
KW - Eggshell protein
KW - Expression
KW - Localization
KW - Molecular cloning
KW - Opisthorchis viverrini
KW - Serodiagnosis
KW - Trematode
UR - http://www.scopus.com/inward/record.url?scp=33749238660&partnerID=8YFLogxK
U2 - 10.1016/j.ijpara.2006.06.012
DO - 10.1016/j.ijpara.2006.06.012
M3 - Article
C2 - 16876169
AN - SCOPUS:33749238660
SN - 0020-7519
VL - 36
SP - 1329
EP - 1339
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 13
ER -