TY - JOUR
T1 - Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea)
T2 - a new nematode in Naja kaouthia from Thailand
AU - Charoennitiwat, Vachirapong
AU - Chaisiri, Kittipong
AU - Kanjanapruthipong, Tapanee
AU - Ampawong, Sumate
AU - Chanhome, Lawan
AU - Vasaruchapong, Taksa
AU - Thaenkham, Urusa
AU - Ratnarathorn, Napat
N1 - Publisher Copyright:
Copyright © 2024 Mahidol University.
PY - 2024/4/4
Y1 - 2024/4/4
N2 - A comprehensive investigation, incorporating both morphological and molecular analyses, has unveiled the existence of a hitherto unknown nematode species, Paracapillaria (Ophidiocapillaria) siamensis sp. nov., residing in the intestine of the monocled cobra, Naja kaouthia, in the central region of Thailand. This study integrates morphological characteristics, morphometric examination, scanning electron microscopy and molecular phylogenetic analysis (COI, 18S rRNA and ITS1 genes). The findings place the newly described species within the subgenus Ophidiocapillaria, elucidating its distinctive characteristics, including a frame-like proximal spicule shape, approximate lengths of 19 000 and 22 500 m with approximate widths of 90 and 130 m for males and females, 39-45 stichocytes, elevated lips without protrusion, a dorsal bacillary band stripe with an irregular pattern of bacillary cells and evidence of intestinal infection. These features serve to differentiate it from other species within the same subgenus, notably Paracapillaria (Ophidiocapillaria) najae De, 1998, a species coexisting P. siamensis sp. nov. in the monocled cobra from the same locality. This study addresses the co-infection of the novel species and P. najae within the same snake host, marking the second documented instance of a paracapillariid species in the monocled cobra within the family Elapidae. The genetic characterization supports the formal recognition of P. siamensis sp. nov. as a distinct species, thereby underscoring its taxonomic differentiation within the Capillariidae family. This research identifies and characterizes the new nematode species, contributing valuable insights into the taxonomy of this nematode.
AB - A comprehensive investigation, incorporating both morphological and molecular analyses, has unveiled the existence of a hitherto unknown nematode species, Paracapillaria (Ophidiocapillaria) siamensis sp. nov., residing in the intestine of the monocled cobra, Naja kaouthia, in the central region of Thailand. This study integrates morphological characteristics, morphometric examination, scanning electron microscopy and molecular phylogenetic analysis (COI, 18S rRNA and ITS1 genes). The findings place the newly described species within the subgenus Ophidiocapillaria, elucidating its distinctive characteristics, including a frame-like proximal spicule shape, approximate lengths of 19 000 and 22 500 m with approximate widths of 90 and 130 m for males and females, 39-45 stichocytes, elevated lips without protrusion, a dorsal bacillary band stripe with an irregular pattern of bacillary cells and evidence of intestinal infection. These features serve to differentiate it from other species within the same subgenus, notably Paracapillaria (Ophidiocapillaria) najae De, 1998, a species coexisting P. siamensis sp. nov. in the monocled cobra from the same locality. This study addresses the co-infection of the novel species and P. najae within the same snake host, marking the second documented instance of a paracapillariid species in the monocled cobra within the family Elapidae. The genetic characterization supports the formal recognition of P. siamensis sp. nov. as a distinct species, thereby underscoring its taxonomic differentiation within the Capillariidae family. This research identifies and characterizes the new nematode species, contributing valuable insights into the taxonomy of this nematode.
KW - Naja kaouthia
KW - Ophidiocapillaria
KW - Paracapillaria siamensis
KW - gastrointestinal helminth
KW - monocled cobra
KW - morphological and molecular analysis
UR - http://www.scopus.com/inward/record.url?scp=85189702637&partnerID=8YFLogxK
U2 - 10.1017/S0031182024000404
DO - 10.1017/S0031182024000404
M3 - Article
AN - SCOPUS:85189702637
SN - 0031-1820
VL - 151
SP - 529
EP - 538
JO - Parasitology
JF - Parasitology
IS - 5
ER -