Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor

Jantana Kampeera; Patiya Pasakon; Chanpen Karuwan; Narong Arunrut; Assawapong Sappat; Sarawut Sirithammajak; Nipaphorn Dechokiattawan; Thitinun Sumranwanich; Parin Chaivisuthangkura; Puey Ounjai; Surang Chankhamhaengdecha; Anurat Wisitsoraat; Adisorn Tuantranont; Wansika Kiatpathomchai

doi:10.1016/j.bios.2019.02.060

Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor

Jantana Kampeera, Patiya Pasakon, Chanpen Karuwan, Narong Arunrut, Assawapong Sappat, Sarawut Sirithammajak, Nipaphorn Dechokiattawan, Thitinun Sumranwanich, Parin Chaivisuthangkura, Puey Ounjai, Surang Chankhamhaengdecha, Anurat Wisitsoraat, Adisorn Tuantranont, Wansika Kiatpathomchai

Research output: Contribution to journal › Article › peer-review

117 Citations (Scopus)

Abstract

Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.

Original language	English
Pages (from-to)	271-278
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	132
DOIs	https://doi.org/10.1016/j.bios.2019.02.060
Publication status	Published - 1 May 2019
Externally published	Yes

Keywords

Detection
LAMP
Point-of-care
Screen-printed graphene electrode
Seafood
Vibrio parahaemolyticus

Access to Document

10.1016/j.bios.2019.02.060

Cite this

Kampeera, J., Pasakon, P., Karuwan, C., Arunrut, N., Sappat, A., Sirithammajak, S., Dechokiattawan, N., Sumranwanich, T., Chaivisuthangkura, P., Ounjai, P., Chankhamhaengdecha, S., Wisitsoraat, A., Tuantranont, A., & Kiatpathomchai, W. (2019). Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor. Biosensors and Bioelectronics, 132, 271-278. https://doi.org/10.1016/j.bios.2019.02.060

@article{f3c99aec4ad24061b6b750bee07f4c11,

title = "Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor",

abstract = "Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.",

keywords = "Detection, LAMP, Point-of-care, Screen-printed graphene electrode, Seafood, Vibrio parahaemolyticus",

author = "Jantana Kampeera and Patiya Pasakon and Chanpen Karuwan and Narong Arunrut and Assawapong Sappat and Sarawut Sirithammajak and Nipaphorn Dechokiattawan and Thitinun Sumranwanich and Parin Chaivisuthangkura and Puey Ounjai and Surang Chankhamhaengdecha and Anurat Wisitsoraat and Adisorn Tuantranont and Wansika Kiatpathomchai",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

month = may,

day = "1",

doi = "10.1016/j.bios.2019.02.060",

language = "English",

volume = "132",

pages = "271--278",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Ltd.",

}

Kampeera, J, Pasakon, P, Karuwan, C, Arunrut, N, Sappat, A, Sirithammajak, S, Dechokiattawan, N, Sumranwanich, T, Chaivisuthangkura, P, Ounjai, P , Chankhamhaengdecha, S, Wisitsoraat, A, Tuantranont, A & Kiatpathomchai, W 2019, 'Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor', Biosensors and Bioelectronics, vol. 132, pp. 271-278. https://doi.org/10.1016/j.bios.2019.02.060

TY - JOUR

T1 - Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor

AU - Kampeera, Jantana

AU - Pasakon, Patiya

AU - Karuwan, Chanpen

AU - Arunrut, Narong

AU - Sappat, Assawapong

AU - Sirithammajak, Sarawut

AU - Dechokiattawan, Nipaphorn

AU - Sumranwanich, Thitinun

AU - Chaivisuthangkura, Parin

AU - Ounjai, Puey

AU - Chankhamhaengdecha, Surang

AU - Wisitsoraat, Anurat

AU - Tuantranont, Adisorn

AU - Kiatpathomchai, Wansika

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.

AB - Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.

KW - Detection

KW - LAMP

KW - Point-of-care

KW - Screen-printed graphene electrode

KW - Seafood

KW - Vibrio parahaemolyticus

UR - http://www.scopus.com/inward/record.url?scp=85062811982&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2019.02.060

DO - 10.1016/j.bios.2019.02.060

M3 - Article

C2 - 30878727

AN - SCOPUS:85062811982

SN - 0956-5663

VL - 132

SP - 271

EP - 278

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -

Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this