TY - GEN
T1 - Suppressive effect of auto-polymerized surgical obturator resin incorporated with vanillin on microbial biofilm
AU - Thaweboon, Sroisiri
AU - Kedcharoen, Pakpoom
AU - Wongsirichat, Natthamet
AU - Thaweboon, Boonyanit
N1 - Publisher Copyright:
© 2020 Trans Tech Publications Ltd, Switzerland.
PY - 2020
Y1 - 2020
N2 - Surgical obturators are important oral prostheses given to patients after surgical treatment to minimize scar contracture and esthetic disfigurement that may affect patients’ functional appearance and psychological health. Poly-methyl methacrylate (PMMA) resin has been the material generally used for fabrication of surgical obturator. However, wearing of this resin appliance can raise a condition for microbial biofilm accumulation and infection of the patients due to the rough surfaces and internal porosities of the resin. Vanillin-incorporated heat-cured and auto-polymerized PMMA resin have been developed and demonstrated to have antimicrobial activity against several types of microorganisms. This study aimed to examine antimicrobial properties of vanillin-incorporated auto-polymerized surgical obturator resin on the biofilm formation of Streptococcus mutans and Candida glabrata. The auto-polymerized PMMA resin samples (Orthocryl® EQ, Germany) were prepared with vanillin incorporation (0.1% and 0.5% vanillin). All samples were coated with sterile saliva and incubated at 37 °C for 60 min. The suspensions of S. mutans ATCC 25715 and C. glabrata ATCC 15126 (107 colony forming unit/mL) were prepared using McFarland standard No.1. Microbial biofilm formation was done in 96-well plate at 37°C for 24 h. The total vital biofilm formation was evaluated by Cell Counting Kit WST-8 (Dojindo Molecular Technologies, USA) at 450 nm. All tests were done in triplicate on three separate circumstances. One-way ANOVA and Tukey’s test were applied for the statistical analysis. A significant decrease of S. mutans biofilm mass was noticed in 0.5% vanillin incorporated resin group compared with a control, resin without vanillin. The percentage of biofilm reduction was 40.8%. No different effect was observed in 0.1% vanillin group. In the case of C. glabrata, no significant biofilm reduction was examined among all groups. In conclusion, the adding of 0.5% vanillin to surgical obturator resin could significantly inhibit biofilm formation of S. mutans, however, the effect was not demonstrated on 0.1% vanillin. C. glabrata was observed to be resistant to vanillin. Using PMMA resin incorporated with 0.5% vanillin, an obturator with antimicrobial property can be applied to prevent dental caries and other systemic diseases in patients after surgical treatment.
AB - Surgical obturators are important oral prostheses given to patients after surgical treatment to minimize scar contracture and esthetic disfigurement that may affect patients’ functional appearance and psychological health. Poly-methyl methacrylate (PMMA) resin has been the material generally used for fabrication of surgical obturator. However, wearing of this resin appliance can raise a condition for microbial biofilm accumulation and infection of the patients due to the rough surfaces and internal porosities of the resin. Vanillin-incorporated heat-cured and auto-polymerized PMMA resin have been developed and demonstrated to have antimicrobial activity against several types of microorganisms. This study aimed to examine antimicrobial properties of vanillin-incorporated auto-polymerized surgical obturator resin on the biofilm formation of Streptococcus mutans and Candida glabrata. The auto-polymerized PMMA resin samples (Orthocryl® EQ, Germany) were prepared with vanillin incorporation (0.1% and 0.5% vanillin). All samples were coated with sterile saliva and incubated at 37 °C for 60 min. The suspensions of S. mutans ATCC 25715 and C. glabrata ATCC 15126 (107 colony forming unit/mL) were prepared using McFarland standard No.1. Microbial biofilm formation was done in 96-well plate at 37°C for 24 h. The total vital biofilm formation was evaluated by Cell Counting Kit WST-8 (Dojindo Molecular Technologies, USA) at 450 nm. All tests were done in triplicate on three separate circumstances. One-way ANOVA and Tukey’s test were applied for the statistical analysis. A significant decrease of S. mutans biofilm mass was noticed in 0.5% vanillin incorporated resin group compared with a control, resin without vanillin. The percentage of biofilm reduction was 40.8%. No different effect was observed in 0.1% vanillin group. In the case of C. glabrata, no significant biofilm reduction was examined among all groups. In conclusion, the adding of 0.5% vanillin to surgical obturator resin could significantly inhibit biofilm formation of S. mutans, however, the effect was not demonstrated on 0.1% vanillin. C. glabrata was observed to be resistant to vanillin. Using PMMA resin incorporated with 0.5% vanillin, an obturator with antimicrobial property can be applied to prevent dental caries and other systemic diseases in patients after surgical treatment.
KW - Biofilm
KW - Resin
KW - Surgical obturator
KW - Vanillin
UR - http://www.scopus.com/inward/record.url?scp=85091663354&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/KEM.862.130
DO - 10.4028/www.scientific.net/KEM.862.130
M3 - Conference contribution
AN - SCOPUS:85091663354
SN - 9783035716962
T3 - Key Engineering Materials
SP - 130
EP - 134
BT - Materials Science
A2 - Hu, Jong Wan
PB - Trans Tech Publications Ltd
T2 - 4th Annual International Conference on Material Science and Engineering Technology, ICMSET 2020
Y2 - 20 March 2020 through 22 March 2020
ER -