TY - JOUR
T1 - The peripheral blood mononuclear cells preparation and the hematology of Varanus salvator
AU - Thanasak, Jitkamol
AU - Tansatit, Tawewan
AU - Taowan, Jarupha
AU - Hirunwiroj, Napawan
AU - Chitthichanonte, Sujit
AU - Wongmack, Teetat
N1 - Publisher Copyright:
© 2022 Thanasak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2022/7
Y1 - 2022/7
N2 - The objectives of this study were to evaluate the proper anticoagulants coated in blood-collecting tube for the peripheral blood mononuclear cells (PBMCs) isolation and to evaluate the proper culture temperature for the Varanus salvator’s PBMCs, in addition, the hematological characteristics also reported. The heparin treated blood (n = 10) and EDTA treated blood (n = 10) from Varanus salvator were obtained for PBMCs evaluation. The PBMCs obtained from the heparin treated blood was significantly higher than that of EDTA treated blood during the culture period (P < 0.05) indicated heparin would be more appropriated anticoagulant for blood collection. The PBMCs cultured under 37◦C and 27◦C were not significantly difference on first three days but 37◦C showed significantly higher effect in the following days (P < 0.05) indicated both temperatures can be used which 37◦C should be an optimal for PBMCs preparation. The peripheral blood cells of Varanus salvator (n = 49) were analyzed for hematological profiles and characteristics which the number of erythrocytes 1.19 ± 0.04 x 1012/L (1.17–1.35 x 1012/L) and WBC 2.41 ± 0.13 x 109/L (2.29–2.81 x 109/L) with absolute differential count of heterophils 0.92 ± 0.02 x 109/L (0.87–0.95 x 109/L), lymphocytes 1.17 ± 0.01 x 109/L (1.15–1.23 x 109/L), azurophils 0.40 ± 0.01 x 109/L (0.37–0.42 x 109/L), basophils 0.000 ± 0.001 x 109/L (0.004–0.011 x 109/L) and monocytes 0.027 ± 0.002 x 109/L (0.028–0.039 x 109/L). These results would play an important role on the cell immunological studies of the Varanus salvator in the future.
AB - The objectives of this study were to evaluate the proper anticoagulants coated in blood-collecting tube for the peripheral blood mononuclear cells (PBMCs) isolation and to evaluate the proper culture temperature for the Varanus salvator’s PBMCs, in addition, the hematological characteristics also reported. The heparin treated blood (n = 10) and EDTA treated blood (n = 10) from Varanus salvator were obtained for PBMCs evaluation. The PBMCs obtained from the heparin treated blood was significantly higher than that of EDTA treated blood during the culture period (P < 0.05) indicated heparin would be more appropriated anticoagulant for blood collection. The PBMCs cultured under 37◦C and 27◦C were not significantly difference on first three days but 37◦C showed significantly higher effect in the following days (P < 0.05) indicated both temperatures can be used which 37◦C should be an optimal for PBMCs preparation. The peripheral blood cells of Varanus salvator (n = 49) were analyzed for hematological profiles and characteristics which the number of erythrocytes 1.19 ± 0.04 x 1012/L (1.17–1.35 x 1012/L) and WBC 2.41 ± 0.13 x 109/L (2.29–2.81 x 109/L) with absolute differential count of heterophils 0.92 ± 0.02 x 109/L (0.87–0.95 x 109/L), lymphocytes 1.17 ± 0.01 x 109/L (1.15–1.23 x 109/L), azurophils 0.40 ± 0.01 x 109/L (0.37–0.42 x 109/L), basophils 0.000 ± 0.001 x 109/L (0.004–0.011 x 109/L) and monocytes 0.027 ± 0.002 x 109/L (0.028–0.039 x 109/L). These results would play an important role on the cell immunological studies of the Varanus salvator in the future.
UR - http://www.scopus.com/inward/record.url?scp=85134808855&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0269108
DO - 10.1371/journal.pone.0269108
M3 - Article
C2 - 35867719
AN - SCOPUS:85134808855
SN - 1932-6203
VL - 17
JO - PLoS ONE
JF - PLoS ONE
IS - 7 July
M1 - e0269108
ER -