TY - JOUR
T1 - Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification
AU - Rueanghiran, Chalalai
AU - Apiwathnasorn, Chamnarn
AU - Sangthong, Pradit
AU - Samung, Yudthana
AU - Ruangsittichai, Jiraporn
PY - 2011
Y1 - 2011
N2 - DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepFl/LepRl the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.
AB - DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepFl/LepRl the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.
KW - COI
KW - Mansonia annulata
KW - Mitochondrial DNA
KW - Primer
UR - http://www.scopus.com/inward/record.url?scp=84857668658&partnerID=8YFLogxK
M3 - Review article
C2 - 22299406
AN - SCOPUS:84857668658
SN - 0125-1562
VL - 42
SP - 1381
EP - 1387
JO - Southeast Asian Journal of Tropical Medicine and Public Health
JF - Southeast Asian Journal of Tropical Medicine and Public Health
IS - 6
ER -